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kb dna substrate  (New England Biolabs)


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    Structured Review

    New England Biolabs kb dna substrate
    Kb Dna Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%BB+dna/Lambda+DNA/pm42913629-185-2-8
    Average 97 stars, based on 1840 article reviews
    kb dna substrate - by Bioz Stars, 2026-11
    97/100 stars

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    Labeling:

    Article Title: Unveiling a missing component of the atypical type IV secretion system required for natural transformation of Helicobacter pylori
    Article Snippet: .. When cultures reached exponential phase (18-24h), OD was normalized to 4 in 20 μL and 200 ng of λ-DNA (New England Biolabs), labelled with Cy3 using Label IT Nucleic Acid Labeling Kit (Mirus Bio) according to manufacturer instructions, were added to the reaction. ..

    Ligation:

    Article Title: Nanoclustering of a plant transcription factor enables strong yet specific DNA binding
    Article Snippet: .. IR7b19 and NSb19 (Table S1) were obtained by annealing Cy5-labeled oligos in a 95 °C heat bath which was gradually cooled overnight. λ-DNA (NEB) templates were biotinylated in batches of 0.2 pmol by annealing primers (Table S2; CosR-bio, 2 pmol; CosL, 20 pmol) at 65 °C followed by ligation (NEB, T4 Ligase) for 2 h at RT, as described in ( ). ..

    Purification:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase
    Article Snippet: In brief, the tissue was removed from the RNAlater solution for lysis and homogenization, and the DNA was collected by transferring the lysate through an AllPrep DNA Mini spin column (Qiagen). cfDNA was extracted from 1 mL of plasma via QIAamp Circulating Nucleic Acid Kit (Qiagen) following the instructions of the protocol. .. 200 ng of λ-DNA (NEB) or mESC gDNA supplemented with 0.1% (w:w) λ-DNA were fragmented with NEBNext dsDNA Fragmentase at 37 °C for 20 min and purified with a DNA Clean & Concentrate Kit (Zymo Research). .. Fragmented λ-DNA was added with a 1% (w:w) 200 bp dsDNA oligo (IDT, Additional file : Table S1) and a 1% (w:w) 164 bp dsDNA oligo as spike-ins (IDT, Additional file : Table S1).

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: In brief, the tissue was removed from the RNAlater solution for lysis and homogenization, and the DNA was collected by transferring the lysate through an AllPrep DNA Mini spin column (Qiagen). cfDNA was extracted from 1 mL of plasma via QIAamp Circulating Nucleic Acid Kit (Qiagen) following the instructions of the protocol. .. Library preparation starting with λ-DNA and mESC gDNA for methylated DNA enrichment in DMN+ 200 ng of λ-DNA (NEB) or mESC gDNA supplemented with 0.1% (w:w) λ-DNA were fragmented with NEBNext dsDNA Fragmentase at 37 °C for 20 mins and purified with a DNA Clean & Concentrate Kit (Zymo Research). .. Fragmented λ-DNA was added with a 1% (w:w) 200 bp dsDNA oligo (IDT, AR TIC LE IN PR ES S Additional file 1: Table S1) and a 1% (w:w) 164 bp dsDNA oligo as spike-ins (IDT, Additional file 1: Table S1).

    Article Title: A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro
    Article Snippet: Gel images were analyzed in ImageLab (BioRad) by manual band calling based on fragment size, and cleavage fractions were determined from relative band intensities. .. Pooled cleavage reactions containing 1.5 μ g of pJET-AAVS1 or λ DNA were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s protocol. .. End-repair and dA-tailing were performed using the NEBNext Companion Module, followed by ligation of bar-coded adapters from the Native Barcoding Expansion kit (EXP-NB104, Oxford Nanopore Technologies).

    Methylation:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: In brief, the tissue was removed from the RNAlater solution for lysis and homogenization, and the DNA was collected by transferring the lysate through an AllPrep DNA Mini spin column (Qiagen). cfDNA was extracted from 1 mL of plasma via QIAamp Circulating Nucleic Acid Kit (Qiagen) following the instructions of the protocol. .. Library preparation starting with λ-DNA and mESC gDNA for methylated DNA enrichment in DMN+ 200 ng of λ-DNA (NEB) or mESC gDNA supplemented with 0.1% (w:w) λ-DNA were fragmented with NEBNext dsDNA Fragmentase at 37 °C for 20 mins and purified with a DNA Clean & Concentrate Kit (Zymo Research). .. Fragmented λ-DNA was added with a 1% (w:w) 200 bp dsDNA oligo (IDT, AR TIC LE IN PR ES S Additional file 1: Table S1) and a 1% (w:w) 164 bp dsDNA oligo as spike-ins (IDT, Additional file 1: Table S1).

    other:

    Article Title: Flourescent protein composition for DNA sequence analysis and method for DNA sequence analysis using same
    Article Snippet: E. coli BL21 (DE3) strain was purchased from Yeastern (Taiwan). λ DNA(NC_001416.1, 48,502 bp) and single-stranded M13mp18 (7,249 bp) were purchased from New England Biolabs (US).

    Translocation Assay:

    Article Title: Nanopore functionality control
    Article Snippet: Voltages were set and currents recorded by custom written LabView software4,20 or with ClampEx (Molecular Devices, USA). .. For translocation experiments we used linearized λ-DNA (New England Biolabs, USA) diluted in the measurement buffer prior to all experiments. ..

    Polymerase Chain Reaction:

    Article Title: A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro
    Article Snippet: Gel images were analyzed in ImageLab (BioRad) by manual band calling based on fragment size, and cleavage fractions were determined from relative band intensities. .. Pooled cleavage reactions containing 1.5 μ g of pJET-AAVS1 or λ DNA were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s protocol. .. End-repair and dA-tailing were performed using the NEBNext Companion Module, followed by ligation of bar-coded adapters from the Native Barcoding Expansion kit (EXP-NB104, Oxford Nanopore Technologies).



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